NK cell–driven IL-2 selectively favors NK expansion. (A) Frequency of GFP expression among NK cells. (B) Cellularity of spleen and LN of 4–6-wk-old NKp46creRosaIL-2 and littermate controls. (C) Representative gating, frequency, and number of splenic NK cells. (D–F) Frequency and number of CD4 Tconv (D), CD8 Tconv (E), and CD4 Treg (F) cells in spleen. (G) Frequency of Foxp3+ cells among total CD4+ T cells. (H) Representative tSNE of high-parameter flow cytometry data from splenic CD4+ (top) and CD8+ (bottom). (I and J) Average frequency of each cluster per mouse for CD4 T cells (I) or CD8 T cells (J). (K and L) Frequency of effector (CD44hiCD62Llo) or central memory (CD44hiCD62Lhi) cells from CD4 Tconv (K) or CD8 Tconv (L) cells. Data pooled from two independent experiments with four to six mice per genotype. Significance was tested by unpaired t test (A–G, K, and L) or two-sample Kolmogorov–Smirnov test (H).
Figure 7.

NK cell–driven IL-2 selectively favors NK expansion. (A) Frequency of GFP expression among NK cells. (B) Cellularity of spleen and LN of 4–6-wk-old NKp46creRosaIL-2 and littermate controls. (C) Representative gating, frequency, and number of splenic NK cells. (D–F) Frequency and number of CD4 Tconv (D), CD8 Tconv (E), and CD4 Treg (F) cells in spleen. (G) Frequency of Foxp3+ cells among total CD4+ T cells. (H) Representative tSNE of high-parameter flow cytometry data from splenic CD4+ (top) and CD8+ (bottom). (I and J) Average frequency of each cluster per mouse for CD4 T cells (I) or CD8 T cells (J). (K and L) Frequency of effector (CD44hiCD62Llo) or central memory (CD44hiCD62Lhi) cells from CD4 Tconv (K) or CD8 Tconv (L) cells. Data pooled from two independent experiments with four to six mice per genotype. Significance was tested by unpaired t test (A–G, K, and L) or two-sample Kolmogorov–Smirnov test (H).

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