DC-driven IL-2 favors Treg expansion. (A) Frequency of GFP expression among DC subsets. (B) Cellularity of spleen and LN of 4–6-wk-old Clec9a^creRosa^IL-2 and littermate controls. (C) Representative gating, frequency, and number of splenic DCs. (D) Frequency of CD8a⁺, CD11b⁺, and CD103⁺ splenic DCs. (E–G) Frequency and number of CD4⁺ Tconv (E), CD8⁺ Tconv (F), and CD4⁺ Treg (G) in spleen. (H) Frequency of Foxp3⁺ cells among total CD4 T cells. (I) Representative tSNE of high-parameter flow cytometry data from splenic CD4⁺ (top) and CD8⁺ (bottom). (J and K) Average frequency of each cluster per mouse for CD4 T cells (J) or CD8 T cells (K). (L and M) Frequency of effector (CD44^hi CD62L^lo) or central memory (CD44^hi CD62L^hi) cells from CD4 Tconv (L) or CD8 Tconv (M) cells. Data pooled from three independent experiments with 8–10 mice per genotype. Significance was tested by unpaired t test (A–H, L, and M) or two-sample Kolmogorov–Smirnov test (I).