IL-2 self-sufficiency drives Treg expansion without altering subset composition. Splenocyte data from 4–6-wk-old Foxp3^cre/wtRosa^IL-2, Foxp3^creRosa^IL-2, or littermate controls from Fig. 3 G. n = 5–15. (A) Average frequency of each cluster per mouse. (B) Heatmap showing differential marker expression in annotated FlowSOM clusters. (C) Average frequency of each Treg subcluster per mouse, from Fig. 3 J. (D) Heatmap showing differential marker expression in annotated FlowSOM Treg subclusters.