T cell–driven IL-2 induces large-scale phenotypic shifts in CD4 and CD8 T cells. (A) Thymic cellularity of 4–6-wk-old CD8^creRosa^IL-2, CD4^creRosa^IL-2, or littermate controls. n = 12–34. (B and C) Representative gating (B) and frequency (C) of thymocyte subsets. n = 9–21. DP, double-positive; SP, single-positive. (D) Frequency of Foxp3⁺ cells among total CD4 T cells. n = 9–21. (E) CD4 T cell data from 4–6-wk-old CD8^cre Rosa^IL2, CD4^cre Rosa^IL2, or littermate controls from Fig. 2 E. Average frequency of each cluster per mouse. (F) Dendrogram showing comparative similarity calculated using cross-entropy distributions from tSNE. (G) Heatmap showing differential marker expression in annotated FlowSOM clusters. (H) Heatmap showing differential marker expression in total CD4⁺ T cells from different strains, showing mouse replicates. (I) CD8 T cell data from 4–6-wk-old CD8^cre Rosa^IL2, CD4^cre Rosa^IL2, or littermate controls from Fig. 2 E. Average frequency of each cluster per mouse. (J) Dendrogram showing comparative similarity calculated using cross-entropy distributions from tSNE. (K) Heatmap of differential marker expression in annotated FlowSOM clusters. (L) Heatmap of differential marker expression in total CD8⁺ T cells from different strains, showing mouse replicates. Data pooled from two or more independent experiments. Significance was tested by one-way ANOVA (A, C, and D).