Comparison of fresh and frozen samples. (a) UMAP dimension reduction after the integration of the frozen dataset, fresh dataset, and dataset from Xue et al. (2020). (b) RNA KNN cluster in Fig. 2 e is represented as a fresh-frozen integrated UMAP. (c) Bar graph of the percentage of each DC and macrophage (MAC) subset in frozen dataset, fresh dataset, and dataset from Xue et al. (2020).