Optimization of annotations through integration with published data. (a) New UMAP dimension reduction after the integration of the three datasets. HCs are healthy skin. (b) The RNA KNN cluster in Fig. 1 e is represented as an integrated UMAP. (c) Surface protein expression is overlaid onto an integrated UMAP dimensional reduction. (d) DC2, DC3, and monocyte feature gene expression shown in Cytlak et al. (2020), overlaid on UMAP. The gene list is the same as in Fig. S1 f. (e) DC and macrophage (Mac) subset signatures from Xue et al. (2020) were shown as a heat map of the specific signature genes expressed. (f) New annotations from the integration of the three datasets. LC, Langarhans cell; MAC, macrophage.