Inhibition of rhesus monkey NK cells and macrophages by B2M^−/−CIITA^−/− hiECs overexpressing rhesus CD47. (A) WT, B2M^−/−CIITA^−/−, and B2M^−/−CIITA^−/− CD47 tg hiECs were challenged with rhesus NK cells using in vitro impedance assays. Rhesus NK cells were used either unstimulated or stimulated with rhesus IL-2. Graphs show mean ± SD and three independent replicates per group and time point; three different E:T ratios are shown. (B) The rhesus CD47 tg was expressed in B2M^−/−CIITA^−/− rhCD47 hiECs, and expression by fluorescence is compared with primary rhesus ECs (representative histogram of two independent experiments). (C)B2M^−/−CIITA^−/− rhCD47 tg hiECs were challenged with unstimulated or rhesus IL-2–stimulated rhesus NK cells. Graphs show mean ± SD and three independent replicates per group and time point; three different E:T ratios are shown. (D) WT, B2M^−/−CIITA^−/−, and B2M^−/−CIITA^−/− rhCD47 tg hiECs were challenged with rhesus macrophages. Graphs show mean ± SD and three independent replicates per group and time point; three different E:T ratios are shown. wt, wild-type.