CD47 is species specific, with no cross-reactivity between mouse and human. (A and B) IFN-γ ELISpot assays were performed with B2m^−/−Ciita^−/− and B2m^−/−Ciita^−/− Cd47 tg miPSCs as target cells and syngeneic C57BL/6 (A) or xenogeneic human primary NK cells as effector cells (B). Cd47 antibody blockade (clone BE0270) was used in some groups, and Yac-1 was used as a control (boxes show 25th to 75th percentile with median, and whiskers show minimum to maximum; 12 independent experiments per miPSC group and 6 for Yac-1 groups, ANOVA with Bonferroni’s post hoc test). (C and D) IFN-γ ELISpot assays were performed with B2M^−/−CIITA^−/− and B2M^−/−CIITA^−/− CD47 tg hiPSCs as target cells and allogeneic human primary NK cells (C) or xenogeneic C57BL/6 NK cells as effector cells (D). CD47 antibody blockade (clone B6.H12) was used in some groups, and K562 was used as a control (boxes show 25th to 75th percentile with median, and whiskers show minimum to maximum, 12 independent experiments per hiPSC group and 6 for K562 groups, ANOVA with Bonferroni’s post hoc test). (E–H) Fluc⁺B2M^−/−CIITA^−/− and B2M^−/−CIITA^−/− CD47 tg hiPSCs were incubated with allogeneic human macrophages (E and F) or xenogeneic C57BL/6 macrophages (G and H), and the BLI signal was quantified (boxes show 25th to 75th percentile with median, and whiskers show minimum to maximum; n = 16 [control], 20 [macrophages], and 9 [Triton X-100] independent samples, ANOVA with Bonferroni’s post hoc test). CD47 antibody blockade was used in some groups. (I–L) Fluc⁺B2m^−/−Ciita^−/− and B2m^−/−Ciita^−/− Cd47 tg miPSCs were incubated with syngeneic C57BL/6 macrophages (I and J) or xenogeneic human macrophages (K and L), and the BLI signal was quantified (boxes show 25th to 75th percentile with median, and whiskers show minimum to maximum; n = 16 [control], 20 [macrophages], and 9 [Triton X-100] independent samples, ANOVA with Bonferroni’s post hoc test). Cd47 antibody blockade was used in some groups.