Cd47 overexpression protects MHC-deficient mouse iPSCs from killing by stimulated NK cells or macrophages in vivo. A 1:1 mixture of CFSE-labeled WT and either B2m−/−Ciita−/− or B2m−/−Ciita−/− Cd47 tg miPSCs was injected into the peritoneum of mice on a syngeneic C57BL/6 background. After 48 h, the ratio of recovered CFSE-positive miPSCs was determined (mean ± SD, triplicates in four animals per group). (A) Recipient WT C57BL/6 mice did not receive additional treatment. (B) A tg CD11b-DT receptor mouse on C57BL/6 background was used to selectively deplete macrophages. (C) The peritoneal cell populations in macrophage-depleted tg CD11b-DT receptor mice were restored by peritoneal cell transfer from WT C57BL/6 mice. (D) Macrophages were pharmacologically depleted in WT C57BL/6 mice using clodronate. (E) In macrophage-depleted mice, peritoneal NK cells were stimulated by peritoneal injections of mouse IL-2. (F) In macrophage-depleted mice, peritoneal NK cells were stimulated by injections of mouse IL-15. (G) In C57BL/6 mice, NK cells were depleted with an anti-NK1.1 depleting antibody. (H) In WT C57BL/6 mice, both macrophages and NK cells were depleted. (I) A 1:1 mixture of CFSE-labeled B2m−/−Ciita−/− and B2m−/−Ciita−/− Cd47 tg miPSCs was injected into WT C57BL/6 mice. wt, wild-type.
Figure 1.

Cd47 overexpression protects MHC-deficient mouse iPSCs from killing by stimulated NK cells or macrophages in vivo. A 1:1 mixture of CFSE-labeled WT and either B2m−/−Ciita−/− or B2m−/−Ciita−/− Cd47 tg miPSCs was injected into the peritoneum of mice on a syngeneic C57BL/6 background. After 48 h, the ratio of recovered CFSE-positive miPSCs was determined (mean ± SD, triplicates in four animals per group). (A) Recipient WT C57BL/6 mice did not receive additional treatment. (B) A tg CD11b-DT receptor mouse on C57BL/6 background was used to selectively deplete macrophages. (C) The peritoneal cell populations in macrophage-depleted tg CD11b-DT receptor mice were restored by peritoneal cell transfer from WT C57BL/6 mice. (D) Macrophages were pharmacologically depleted in WT C57BL/6 mice using clodronate. (E) In macrophage-depleted mice, peritoneal NK cells were stimulated by peritoneal injections of mouse IL-2. (F) In macrophage-depleted mice, peritoneal NK cells were stimulated by injections of mouse IL-15. (G) In C57BL/6 mice, NK cells were depleted with an anti-NK1.1 depleting antibody. (H) In WT C57BL/6 mice, both macrophages and NK cells were depleted. (I) A 1:1 mixture of CFSE-labeled B2m−/−Ciita−/− and B2m−/−Ciita−/− Cd47 tg miPSCs was injected into WT C57BL/6 mice. wt, wild-type.

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