T cell–dependent B cell responses in mice lacking SLAMF1, SLAMF5, or SLAMF6, alone or in combination. Related to Fig. 10. (A–F) Same as Fig. 10 H, except that SLAMF1 KO (A and B), SLAMF5 KO (C and D), and SLAMF6 KO (E and F) were studied. Numbers of GC B cells, NIP⁺IgM⁺ GC B cells, and NIP⁺IgG1⁺ GC B cells were determined (A, C, and E). Levels of anti-NP IgM and IgG1 were also determined (B, D, and F). (G) Same as Fig. 10 I, except that numbers of GC B cells, NIP⁺IgM⁺ GC B cells, and NIP⁺IgG1⁺ GC B cells were assessed. For A, WT, n = 4; and SLAMF1 KO, n = 8; data are pooled from two experiments. For B, WT, n = 7; and SLAMF1 KO, n = 11. Data are from one experiment. For C, WT, n = 5; and SLAMF5 KO, n = 7. Data are pooled from two experiments. For D, WT, n = 8; and SLAMF5 KO, n = 8. Data are pooled from two experiments. For E, WT, n = 5; and SLAMF6 KO, n = 4. Data are from one experiment. For F, WT, n = 6; and SLAMF6 KO, n = 11. Data are pooled from two experiments. For G, WT, n = 7; and SLAMF1,5,6 tKO, n = 7. Data are pooled from two experiments. Each symbol represents one mouse. *, P < 0.05; **, P < 0.01 (two-tailed Student’s t test). Data are shown as mean ± SEM. ns, not significant; SPC, spleen cells.