Macrophages are dispensable for exclusion of splenic cDC2s from the MZ. (A and B) Frequencies of DCIR2⁺ cDC2s in DCs (A, left) or in vivo anti-CD45-PE labeling of cDC2s (A, right) and representative distribution patterns of MARCO⁺ MZMs (green) and DCIR2⁺ cDC2s (red) relative to IgD⁺ B cells (blue) in spleens (B) 2–3 wk after clodronate liposome (Lipo) or control (Con) treatment. (C) In vivo anti-CD45-PE labeling of MZ B cells in mice in A. (D and E) Frequencies of DCIR2⁺ cDC2s in total DCs (D, left) or in vivo anti-CD45-PE labeling of cDC2s (D, right) and representative distribution patterns of CD169⁺ MMMs (green) or DCIR2⁺ cDC2s (red) relative to IgD⁺ B cells (blue) in spleens (E) 2–3 d after diphtheria toxin (DT) treatment in CD169^DTR/+ and control mice. (F) In vivo anti-CD45-PE labeling of MZ B cells in mice in D. Each symbol represents one mouse, and lines denote means. One of three independent experiments with similar results is shown. Sections are representative of of multiple spleen cross sections from six mice of each type. Scale bar, 200 µm.