Representative distribution patterns of in vivo pulse-labeled DCIR2⁺ cDC2s. (A) Representative FACS profiles displaying gate between CD4 and DCIR2 in DCIR2⁺ CD8⁻ cDC2 cells. (B) FACS profiles and frequencies of in vivo anti-CD45-PE–labeled CD8⁺ cDC1s in mice of the indicated ages. Each symbol represents one mouse, and lines denote means. One of three independent experiments with similar results is shown, in which each group had at least three mice. (C) In vivo antibody labeling of DCIR2⁺ cDC2s (red) relative to IgD⁺ B cells (blue) in spleens 3 min after i.v. injection of PE-conjugated anti-CD11c (green). Blood-exposed DCIR2⁺ DCs (cells that have bound CD11c-PE) appear yellow. Multiple images are stitched to obtain the full spleen view. Scale bar, 500 µm. (D and E) Representative views of spleens 3 min after i.v. injection of PE-conjugated anti-CD45 (green) from the mice of the indicated ages (D) or spleens of adult mice 4 h after saline or FTY720 treatment (E). Blood-exposed DCIR2⁺ DCs (cells that have bound CD45.2-PE) appear yellow. Scale bar, 200 µm. Sections are representative of multiple cross sections from five mice of each type. **, P < 0.01.