snRNA-seq analyses of ARH neurons in the adult AgRP-Cre; Sun1-GFP mice. (A) Flow cytometry sorting GFP positive nuclei from 15 adult AgRP-Cre; Sun1-GFP mice. (B) Heatmap of normalized HTO values based on HTOdemux. Doublets (or multiplets) express more than one HTO signature. (C) tSNE embedding of the HTO dataset revealed 15 singlet clusters. (D) Transcriptome-based dimensionality reduction using UMAP identified neuronal and non-neuronal clusters. (E) Heatmap of the marker genes for the six identified clusters in the UMAP aggregate of all cells. (F) Violin plot of the key marker genes in the six identified clusters.