Chimeric hIFNγR-hIL-12R receptor (CC12R) design and expression in human primary NK cells. Purified frozen human primary NK cells were cultured for 3 d with or without lentiviral particles containing the CC12R construct and IL-2 (3 U/ml). CC12R expression was evaluated by anti-myc-tag (first CC12R chain) and anti-FLAG (second CC12R chain). Expression was evaluated relative to donor-matched control samples (CC12R−). Cell viability was assessed using a near-infrared live/dead marker. Four donors (n = 4). Mean ± SD. Paired t test, parametric; *, P < 0.05. (A) (A.i) Expression of CC12R after 3 d of transduction. (A.ii) Percentage of CC12R+ cells evaluated by FLAG expression. (A.iii) Relative cell viability. (B) Influence of IFNγ stimulation on cell proliferation of CC12R⁺ cells.