Molecular differences between infant and adult Tconv cells. (A and B) Low-input RNA-seq was performed on 1,000 LN naive Tconv cells from infant and adult mice. (A) Differential expression shown as a volcano plot (fold change versus P value). TCR activation signature genes are highlighted in purple. Number of genes and χ²-test P value are indicated. (B) GSEA showing differentially enriched hallmark gene sets. Data are average values of three biological replicates. (C) Nur77-GFP expression on naive T cells from mice of different ages (left) and how they correlate with frequencies of RORγ⁺ pTreg cells following Tconv cell transfer from mice of different ages (n ≥ 3; RORγ⁺ frequency data taken from Fig. 5 A). (D) Mean fluorescence intensities of CD5 and c-Maf on naive T cells, and frequencies of CD69^hi and ST2⁺ among naive T cells from mice of different ages (n ≥ 3). Data are pooled from three independent experiments. Means ± SD. MFI, mean fluorescence intensity; wo, weeks old; Tg, transgenic.