Disruption of single and multiple genes in human monocyte-derived macrophages. (A) Histograms depicting cell surface B2M, CD14, and CD81 protein levels in monocyte-derived macrophages as measured by flow cytometry. (B) Quantification of gene deletion as measured by flow cytometry. Data are presented as mean ± SD (n = 3) and representative of three independent donors. (C) Assessment of gene editing efficiency by Sanger sequencing 7 d after nucleofection. Data are mean ± SEM (n = 3) and representative of three independent donors.