Figure 9. Loss of Ptsn expression... | Rockefeller University Press

Figure 9.

Loss of Ptsn expression protects mice from colitis. Conventional T cells (1 × 106) from WT or Ppp1r18−/− mice were adoptively transferred into Rag1−/− mice. (A) Changes in body weight are plotted. Values are normalized as a percentage of the original weight. Two-way ANOVA with Bonferroni post-test. ***, P < 0.001. (B) Representative H&E staining of distal colon sections wrapped like Swiss rolls from Rag1−/− mice injected with conventional T cells from WT or Ppp1r18−/− mice sacrificed on day 100. Scale bars: 1 mm or 200 μm. (C) Histology score. Two-tailed t test; ***, P < 0.001. (D) mRNA expression of IL-1β, TNF-α, IL-6, IFN-γ, and IL-17A in distal colon tissues on day 100. Results are normalized to expression of GAPDH. Mean ± SEM are plotted. Two-tailed t test; **, P < 0.01; ***, P < 0.001.

Loss of Ptsn expression protects mice from colitis. Conventional T cells (1 × 10⁶) from WT or Ppp1r18^−/− mice were adoptively transferred into Rag1^−/− mice. (A) Changes in body weight are plotted. Values are normalized as a percentage of the original weight. Two-way ANOVA with Bonferroni post-test. ***, P < 0.001. (B) Representative H&E staining of distal colon sections wrapped like Swiss rolls from Rag1^−/− mice injected with conventional T cells from WT or Ppp1r18^−/− mice sacrificed on day 100. Scale bars: 1 mm or 200 μm. (C) Histology score. Two-tailed t test; ***, P < 0.001. (D) mRNA expression of IL-1β, TNF-α, IL-6, IFN-γ, and IL-17A in distal colon tissues on day 100. Results are normalized to expression of GAPDH. Mean ± SEM are plotted. Two-tailed t test; **, P < 0.01; ***, P < 0.001.