Alternative generation of Ppp1r18 ^−/− mice. (A) Generation of Ppp1r18^−/− mice by deleting the entire coding sequence of exon1. Four sgRNAs targeting the exon1 were used. The edited sequence of the repaired Ppp1r18^−/− allele is shown. (B)Ppp1r18^−/− mice manifested loss of both α- and β-Ptsn isoforms. Ptsn expression in splenocytes was assayed by immunoblotting using an antibody that reacts with the C-terminus of Ptsn and recognizes both α- and β-Ptsn isoforms. Source data are available for this figure: SourceData FS3.