Ptsn contributes to lymphocyte migration. WT, Ptsn-null, or β-Ptsn–reconstituted Ptsn-null Jurkat T cells described in Fig. 3 were adhered to VCAM-1–coated 24-well glass-bottom plates and were imaged every min for 30 min. (A) Scattergrams of individual cell trajectories of Jurkat WT, Ptsn KO, and β-Ptsn rescue cells. (B) Average speed of Jurkat WT, Ptsn KO, and β-Ptsn rescue cells. Data are presented as mean ± SEM with n = total number of cells tracked (n = 258 WT, 250 KO, and 270 rescue). Significance was tested with one-way ANOVA using multiple comparisons with a Tukey post-test. ***, P < 0.001; ****, P < 0.0001.