Figure S4.
Characterization of larval thyrocyte proliferation in transgenic RET thyroid, and histologic characterization of thyroid tumors arising in transgenic RET adult zebrafish. (A–F) Transgenic zebrafish tg:TdTomato (A–C) were compared to tg:CCDC6-RET; tgTdTomato (D–F) by confocal microscopy at 5 dpf. Larval animals were stained with BrDU to identify cells in S phase (B and E). Merged images (C and F) allow identification of TdTomato + thyrocytes that costained with BrdU, as noted by arrowheads. Representative images are shown. For A–F, scale bar = 50 μm. (G) H&E staining performed on tg:CCDC6-RET transgenic zebrafish with tumors identifies invasion of skeletal muscle (noted by arrow); scale bar = 2 mm. (H) High-powered photomicrograph of CCDC6-RET transgenic tumor identifies mitotic cells in thyroid tumor (noted by arrows); scale bar = 100 μm. This image is also used in Fig. 4 E.

Characterization of larval thyrocyte proliferation in transgenic RET thyroid, and histologic characterization of thyroid tumors arising in transgenic RET adult zebrafish. (A–F) Transgenic zebrafish tg:TdTomato (A–C) were compared to tg:CCDC6-RET; tgTdTomato (D–F) by confocal microscopy at 5 dpf. Larval animals were stained with BrDU to identify cells in S phase (B and E). Merged images (C and F) allow identification of TdTomato + thyrocytes that costained with BrdU, as noted by arrowheads. Representative images are shown. For A–F, scale bar = 50 μm. (G) H&E staining performed on tg:CCDC6-RET transgenic zebrafish with tumors identifies invasion of skeletal muscle (noted by arrow); scale bar = 2 mm. (H) High-powered photomicrograph of CCDC6-RET transgenic tumor identifies mitotic cells in thyroid tumor (noted by arrows); scale bar = 100 μm. This image is also used in Fig. 4 E.

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