Myeloid HIFs prevent cardiac rupture after MI. (A) Survival of mice with myeloid-specific deletion of both Hif1 and Hif2 (mHIF1/2^−/−) or controls after MI. n = 6–7 mice/group pooled from two independent experiments. **, P < 0.01 by log-rank (Mantel–Cox) test. (B) Evidence of cardiac rupture in mHIF1/2^−/− mice compared with controls after MI. Scale bars represent 100 µm (left panel) and 50 µm (right panel). n = 6–7 mice/group pooled from two independent experiments. ***, P < 0.001 by two-tailed, unpaired t test. (C) Absolute number of infarct-associated ⍺SMA⁺ myofibroblasts 3 d after MI in mice with myeloid-specific deletion of Hif1 (mHIF1^−/−), Hif2 (mHIF2^−/−), both Hif1 and Hif2 (mHIF1/2^−/−), or controls. n = 4–5 mice/group pooled from two independent experiments. *, P < 0.05 by one-way ANOVA followed by Tukey’s test. (D) Cardiac macrophage apoptosis as measured by Annexin staining after MI. Black contour plots represent FMO staining controls. n = 8 mice/group pooled from three independent experiments. ***, P < 0.001 by one-way ANOVA followed by Tukey’s test. (E) Infarct-associated cellular responses in mHIF1/2^−/− mice or controls after MI. n = 4–9 mice/group pooled from more than three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA followed by Tukey’s test. All data are presented as mean ± SEM.