A thymocyte emigration defect in adult CD2-iCre;Lsd1^fl/fl mice. (A) CD4 versus CD8 surface staining profiles of total LN cells from 8-wk-old Lsd1^fl/fl and CD2-iCre;Lsd1^fl/fl mice. Numbers in each gate are the percentages of total cells. (B) Number of LN CD4⁺ and CD8⁺ T cells in 8-wk-old Lsd1^fl/fl and CD2-iCre;Lsd1^fl/fl mice. (C) CD62L versus CD44 surface staining of gated CD4⁺ or CD8⁺ T cells from Lsd1^fl/fl and CD2-iCre;Lsd1^fl/fl mice showing CD44^hi “memory-like” phenotype of T cells in CD2-iCre;Lsd1^fl/fl mice. (D) Phenotype of thymocytes from adult Lsd1^fl/fl and CD2-iCre;Lsd1^fl/fl mice. Numbers in each gate are percentages of total cells. (E) Staining of immature CD4⁺CD24^hi and mature CD4⁺CD24^lo thymocytes for S1pr1. (F) Percentage and number of γδTCR⁺ thymocytes in adult Lsd1^fl/fl and CD2-iCre;Lsd1^fl/fl mice. *, P < 0.05; **, P < 0.01; ***, P < 0.005; unpaired t test. Data are represented as mean ± SEM. Results shown in A–E are representative of three or more experiments.