Validation scheme for dCLIP analysis cutoff and selection of targets based on intersection of dCLIP and TargetScan. (A and B) Total number of genes containing an miR-221/222 seed sequence (6-mer or better) in a region with more binding for flox library (aqua) or Δ library (red) or genes having a region for both genotypes (white) when filtering on a dCLIP internal parameter of 1 (A) or 10 (B). (C) Of the 343 genes from B (aqua + white), those that intersect with the 290 genes predicted to be miR-221/222-3p targets by TargetScan (yellow) were selected as siRNA screen candidates (white).