Loss of miR-221/222 decreases the number of plasma cells (PCs) and IgE⁺ PCs in a pan–B cell activation model of goat anti-IgD immunization. (A) OD plots for NP-specific isotype antibodies measured in serum 14 d after immunization with NP-OVA in alum. Data are from three independent immunizations with at least four mice per genotype. (B) ELISA data for total IgE in mice challenged with HDM for a 2-wk protocol from three independent experiments with at least four mice per group. (C) Representative flow plot of the LN cell environment showing PC (CD19^int, CD138⁺) gated on intracellular IgE⁺ fraction. (D) Frequency of PCs (CD19^int, CD138⁺ cells of living singlet). (E) Frequency of IgE⁺ PCs. (F) Numbers of PCs. (G) Numbers of IgE⁺ PCs. A combination of two independent experiments with at least four mice per genotype per experiment is shown. All statistics are from a two-tailed Student’s t test. *, P < 0.05; **, P < 0.01.