Extended validation of model, transfection method, and miRNA candidates. (A) Flow cytometry histograms and quantification of CD45 expression on B cells stimulated in conditions outlined in Fig. 1 A and transfected with CD45 siRNA or control siRNA. Representative of three independent experiments. MFI, mean fluorescence intensity. (B) Follow-up rescreen of top 10 and bottom 10 Z-score miRNAs from original screen, transfected in duplicate over two replicates.