Figure 1.
VP1-2 DUB activity is the major HSV1 IFN antagonist in mixed brain cell cultures.(A–D) Isolated mixed murine brain cells were infected with HSV1 (WT), VP1-2 (ΔDUB), ΔICP0, ΔICP27 (all KOS strain) and HSV1 (WT), ΔICP34.5, ΔUL46 (all F strain; MOI 3) for 8 h. Total RNA was isolated, and mRNA levels of Ifnb, Cxcl10, Isg15, and Il6 were analyzed by quantitative RT-PCR. The mRNA data were normalized to β-actin mRNA levels and are displayed as relative levels compared with HSV1 (WT) values (n = 3). (E)Ifnar1−/− MEFs were infected with WT and ΔDUB HSV1 (KOS strain) for 24 h at MOI 1. Viral loads in the supernatants were determined by plaque assay. P values were calculated using a two-tailed unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001; ns, not significant. Error bars represent standard deviation.

VP1-2 DUB activity is the major HSV1 IFN antagonist in mixed brain cell cultures. (A–D) Isolated mixed murine brain cells were infected with HSV1 (WT), VP1-2 (ΔDUB), ΔICP0, ΔICP27 (all KOS strain) and HSV1 (WT), ΔICP34.5, ΔUL46 (all F strain; MOI 3) for 8 h. Total RNA was isolated, and mRNA levels of Ifnb, Cxcl10, Isg15, and Il6 were analyzed by quantitative RT-PCR. The mRNA data were normalized to β-actin mRNA levels and are displayed as relative levels compared with HSV1 (WT) values (n = 3). (E)Ifnar1−/− MEFs were infected with WT and ΔDUB HSV1 (KOS strain) for 24 h at MOI 1. Viral loads in the supernatants were determined by plaque assay. P values were calculated using a two-tailed unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001; ns, not significant. Error bars represent standard deviation.

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