TNF-α treatment prevents loss of H3H9me3 at intronic L1Md, HSC gene repression, and HSC loss of function upon IR. (A) Integrative genomic viewer visualization of H3K9me3 enrichment obtained by ChIP-seq and CUT&Tag M-MRA analysis at two described loci as indicated: (left) chr2:39209585–39320316; (right) chr6:5271421–5288640 (Bulut-Karslioglu et al. 2014). (B) Enrichment plots in IR vs. NIR and in IR + TNF-α vs. IR conditions obtained from GSEA for low-output and LT-HSC signatures. (C and D) Gating strategy to evaluate donor-derived GFP⁻ vs. recipient GFP⁺ cells in peripheral blood (C) and bone marrow HSCs (D). FDR, false discovery rate; NES, normalized enrichment score.