TNF-α treatment prevents loss of NFKB1 and HSC gene repression in vitro. (A and B) Representative images and quantification of NFKB1 protein mean IF intensity using the monoclonal anti-NFKB1 (p50) antibody clone from Santa Cruz Biotechnology. Bars, 5 µM. Each dot represents a cell. Results are expressed as fold change from the mean value of the NIR condition from two independent experiments and represented as means ± SEM. t test for A or one-way ANOVA with Tukey’s multiple comparison test for B. (C)Ttc8 and Mapre2 mRNA expression evaluated by qRT-PCR. Ct values were normalized to Rpl32 and Hprt. Results are expressed as fold change from the mean value of the NIR condition and represented as means ± SEM from two independent experiments. One-way ANOVA Tukey’s multiple comparison test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.