Irradiation induces a strong deregulation of the HSC transcriptome. (A) Volcano plot of the DEGs between IR and NIR conditions. The horizontal axis represents the log₂ fold change (FC) and the vertical axis the –log₁₀(P value). Significantly (P < 0.05) upregulated (red) and downregulated (green) genes are shown. (B) Repartition of total (P < 0.05) DEG (gray) and downregulated (green) or upregulated (red) genes in IR vs. NIR according to their fold change. (C–H) GSEA analysis using Hallmark gene sets. Significant (P < 0.05) gene sets gained (C) or lost (D) in the IR condition compared to NIR. Log₁₀(P value) is set to 4 when P < 0.001. Enrichment plots for TNF-α signaling via NF-κB hallmark (E), HSC prosurvival TNF-α (F), and GMP TNF-α (G) gene signatures. (H) Enrichment plots for LT-HSC; low-output and dormant vs. activated HSC signatures. FDR, false discover rate; NES, normalized enrichment score.