Figure 1. iv8 binding to inferred germline VRC01-class BCRs. (A) iv8 (black circles) and iv1 (purple triangles) binding to the indicated recombinant inferred-germline (gl), mutated (mt), or chimeric human monoclonal antibodies using BLI. Data points depict the maximum binding response (R Max) of the mAb following a 500-s association step. Each point represents the R Max of an independent replicate (n = 3). Lines indicate arithmetic means; error bars indicate SEM; HC and LC indicate IgHs and IgLs, respectively. (B) Structure of iv8 scFv bound to germline VRC01 scFv. The iv8 IgH and IgLs are shown in dark and light gray, respectively. Germline VRC01 IgH and IgLs are shown in beige and pink, respectively. (C) Distribution of BSA on glVRC01 upon iv8 binding. BSA on light-chain regions is shown in pink; BSA on heavy-chain regions is shown in beige.
Figure 1.

iv8 binding to inferred germline VRC01-class BCRs. (A) iv8 (black circles) and iv1 (purple triangles) binding to the indicated recombinant inferred-germline (gl), mutated (mt), or chimeric human monoclonal antibodies using BLI. Data points depict the maximum binding response (R Max) of the mAb following a 500-s association step. Each point represents the R Max of an independent replicate (n = 3). Lines indicate arithmetic means; error bars indicate SEM; HC and LC indicate IgHs and IgLs, respectively. (B) Structure of iv8 scFv bound to germline VRC01 scFv. The iv8 IgH and IgLs are shown in dark and light gray, respectively. Germline VRC01 IgH and IgLs are shown in beige and pink, respectively. (C) Distribution of BSA on glVRC01 upon iv8 binding. BSA on light-chain regions is shown in pink; BSA on heavy-chain regions is shown in beige.

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