Figure 1.
Anti-MAYV mAbs neutralize strains from both D and L genotypes. (A) Viral strains were subjected to next-generation sequencing. A phylogenetic tree was generated using a Jukes–Cantor genetic distance model by aligning structural gene nucleotide sequences for each MAYV strain. (B) Serial dilutions of mAbs were incubated with 102 FFU of the indicated MAYV strain, representing either genotypes D (top) or L (bottom), before inoculation of Vero cells. Cells were overlaid with methylcellulose and incubated for 18 h. Viral foci were stained, counted, and plotted relative to a no-antibody control. Data are representative of two experiments performed in triplicate. Error bars represent SD within one experiment.
