Figure 1. Induction of cleaved caspase-11 fragment in macrophages in response to cytoplasmic LPS. (A) Schematic of caspase-11 representing the CARD domain and large and small catalytic subunits. Predicted cleavage sites D59, D80, and D285 are indicated. Asterisk (*) marks critical cysteine residue in caspase-11 catalytic site (C254). M61 is a predicted alternative start site. (B and C) BMDMs were treated with 1 µg/ml Pam3CSK4 for 5 h before stimulation. (B) Immunoblot of caspase-11, GSDMD, and actin in combined cell extract (ext) and supernatant (sup) from WT BMDMs 30, 60, and 120 min after LPS electroporation or control. (C) LDH release time course in WT BMDMs 30, 60, and 120 min after LPS stimulation from cells in B. Data are presented as mean ± SD (n = 3) in C and representative of at least two independent experiments (B and C).
Figure 1.

Induction of cleaved caspase-11 fragment in macrophages in response to cytoplasmic LPS. (A) Schematic of caspase-11 representing the CARD domain and large and small catalytic subunits. Predicted cleavage sites D59, D80, and D285 are indicated. Asterisk (*) marks critical cysteine residue in caspase-11 catalytic site (C254). M61 is a predicted alternative start site. (B and C) BMDMs were treated with 1 µg/ml Pam3CSK4 for 5 h before stimulation. (B) Immunoblot of caspase-11, GSDMD, and actin in combined cell extract (ext) and supernatant (sup) from WT BMDMs 30, 60, and 120 min after LPS electroporation or control. (C) LDH release time course in WT BMDMs 30, 60, and 120 min after LPS stimulation from cells in B. Data are presented as mean ± SD (n = 3) in C and representative of at least two independent experiments (B and C).

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