Figure 1.
Figure 1. Characterization of Cre recombinase expression in UBC-Cre/ERT2 mice. (A) Gt(ROSA)26Sortm1Sor/J Cre-reporter mice were bred with UBC-Cre/ERT2 mice to monitor expression of Cre recombinase in the brain, as detected by expression of β-galactosidase. Expression of LacZ, as detected by X-Gal, in P15 brains was sporadic and not readily detected. LacZ was detectable at P45 and was more prominent at P90 in compound mice. Bar, 200 µm. (B) Confocal staining was conducted to monitor the expression of β-galactosidase in neurons (labeled by NeuN antibody), microglia (Iba1 antibody), or astrocytes (Smi22 for GFAP) in P90 compound mice or control littermates. Bar, 100 µm.

Characterization of Cre recombinase expression in UBC-Cre/ ERT2 mice. (A) Gt(ROSA)26Sortm1Sor/J Cre-reporter mice were bred with UBC-Cre/ERT2 mice to monitor expression of Cre recombinase in the brain, as detected by expression of β-galactosidase. Expression of LacZ, as detected by X-Gal, in P15 brains was sporadic and not readily detected. LacZ was detectable at P45 and was more prominent at P90 in compound mice. Bar, 200 µm. (B) Confocal staining was conducted to monitor the expression of β-galactosidase in neurons (labeled by NeuN antibody), microglia (Iba1 antibody), or astrocytes (Smi22 for GFAP) in P90 compound mice or control littermates. Bar, 100 µm.

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