Hsp90β is up-regulated in reactive astrocytes in the hippocampus of HS and mouse models of TLE and FS. (A) Immunohistochemical staining for Hsp90β in the DG, CA1, and CA3 region of autopsy control, nonHS, and HS. Arrows indicate glia-likes cells in the sclerotic hippocampus (left; autopsy, n = 5; nonHS, n = 5; HS, n = 7). Bar, 50 µm. Quantification of Hsp90β⁺ glia-like cells (right; ***, P < 0.001, Student's t test). (B) Representative images of the hippocampus from HS immunostained with Hsp90β (red), with an astrocyte-specific antibody GFAP (green), or with microglia-specific antibody Iba1 (green). Merged images revealed co-localization of Hsp90β with GFAP in astrocytes and a lack of co-localization of Hsp90β with Iba1 in microglia. Bar, 10 µm. (C) Immunofluorescence of Hsp90β and GFAP in the sclerotic hippocampus of mice at 4 wk after SE and in the saline injected control hippocampus (left). Quantification of Hsp90β⁺ GFAP cells (right; n = 4 mice per group). Bar, 25 µm. (D) Immunofluorescence of Hsp90β and GFAP in the hippocampus of mice 5 d after experimental prolonged FS (left). Cell counting was performed under 40× magnification. Quantification of Hsp90β⁺ GFAP cells (right; n = 4 mice per group; ***, P < 0.001, Student's t test). Bar, 15 µm. Data are representative of two independent experiments.