CD19⁻ PCs exhibit features of advanced maturity and are enriched for rotavirus-specific clones. (A) The percentage of CD95⁺ cells was determined relative to isotype control by flow cytometric analysis. n = 5; Whipple procedure. (B) The mean fluorescence intensity (MFI) of Bcl2 relative to isotype control staining (ΔMFI) on PC subsets was determined by flow cytometric analysis and presented as the fraction of total value for each subject. n = 12; Whipple procedure. (C–F) The percentage of Ki67⁺ (n = 9), BCMA⁺ (n = 10), CD56⁺ (n = 14), and CD28⁺ (n = 7) was determined relative to isotype control staining in SI resections (Whipple procedure and Ptx). (G) Rotavirus-reactive PCs were detected using eGFP-labeled VLPs relative to eGFP by flow cytometric analysis (SI resections from Whipple procedure [n = 3] or Ptx [n = 4]; median age 49 yr; range 7–74 yr; 2 female). Data points represent individual subjects. Red bars indicate mean values. FSC, forward side scatter. *, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001; one-way RM-ANOVA.