The effect of lipin-2 in the response to LPS in mice. (A) WT and Lpin2^−/− mice (n = 5) were intraperitoneally injected with 10 mg/kg of LPS or PBS. 3 h later, indicated cytokines were quantified in serum using specific ELISA. Expression levels of the indicated genes were analyzed in liver (B) or spleen (C) by RT-qPCR. (D) Proposed scheme depicting lipin-2 effects on priming and activation of the inflammasome. Data are shown as means ± SD, and experiments shown are representative of at least two independent ones analyzed in triplicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001, by Student’s t test.