STING N153S mice exhibit altered immune cell signaling. (A and B) Splenocytes were harvested from 4–6-mo-old WT and STING N153S mice and analyzed by CyTOF. (A) Representative SPADE plots of phospho-TBK1 (top) and phospho-S6 (bottom) in WT and STING N153S splenocytes. Size of nodes reflects the cell number, and color reflects transformed median intensities of the phosphoprotein signal. (B) Heat maps depicting calculated arcsinh ratios of median intensities of phosphoprotein signal in WT and STING N153S splenocytes. Data were pooled from two independent experiments with n = 6 mice. *, P < 0.05; **, P < 0.005 by Mann–Whitney test.