WT and STING N153S immune cell populations. WT and STING N153S splenocytes were analyzed by CyTOF. (A) viSNE map of WT and STING N153S splenocytes illustrating color-coded cell populations that clustered based on cell surface marker expression. (B) viSNE map indicating the level of CD3 marker expression in immune cell populations defined in A. (C) Density plots of immune cell populations defined in A. (D–I) Total number of CD45⁺ (D), CD19⁺ (E), CD3⁺ (F), CD4⁺ (G), CD8⁺ (H), and Ly6G⁺ (I) cells in WT littermate control and STING N153S splenocytes. Horizontal bars represent the mean of n = 6 samples from two independent experiments. **, P < 0.005 by Mann-Whitney test. (J and K) Heat maps illustrating the calculated arcsinh ratios of median intensities of CD25, CD11b, and CD44 expression in CD4⁺ (J, left), CD8⁺ (J, right), NK1.1⁺ (K, left), and CD3⁺CD4⁻CD8⁻ (CD3⁺DN; K, right) splenocytes. *, P < 0.05; **, P < 0.005 by Mann-Whitney test.