Increased IgM autoantibodies in the serum of STING N153S mice. Immunoglobulin analysis of WT and STING N153S mouse serum (n = 9). (A and B) Quantitation of total IgG (A) and total IgM (B). (C) Heat maps of IgG and IgM autoantibodies against autoantigens. n = 9 per genotype. ****, P < 0.0001; ns, not significant. Data are the result of two independent experiments with four to five samples per experiment and were analyzed by Mann–Whitney test. Error bars represent SEM.