RANK controls intrathymic regulators of promiscuous gene expression. (A) Confocal images of thymus sections stained for Aire (green), ERTR5 (blue), and Fezf2 (red). Bars, 10 µm. Data represent three experiments, n ≥ 6. (B) Aire and Fezf2 in Ly51⁻ mTECs in dGuo FTOCs treated as indicated. Graph shows percentage Aire⁺Fezf2⁺ mTECs. Data from at least three separate experiments. (C and D) qPCR of indicated genes in anti-RANK/anti-LTβR stimulated dGuo FTOCs. Data from at least two independent experiments. Error bars represent SEM.