LTβR deletion in thymic epithelium corrects the autoimmunity in germline Ltbr^−/− mice. (A) LTβR in WT EpCAM1⁺ TECs, Ly51⁺ cTECs, Ly51⁻MHCII^loCD80^lo mTEC^lo cells, and Ly51⁻MHCII^hiCD80^hi mTEC^hi cells; gray histogram is isotype control staining. (B) Anti-LTβR staining in TECs from indicated strains. Data represent two experiments, n ≥ 4 mice. (C) Liver sections from mice at 8–12 wk of age. Arrow indicates lymphocytic infiltrates. Bars, 100 µm. (D) Quantitation of infiltrates in B. Data from ≥3 mice from two experiments. (E) WT sections incubated with 1/80 sera to detect autoantibodies (green); DAPI in red; staining on stomach shown as an example. Bars, 100 µm. (F) Quantification of autoantibody staining in stomach. Data represent at least two experiments, n ≥ 5 mice. (G) Summary of autoantibody detection in various tissues. Each segment represents one mouse; black denotes positive staining. Error bars indicate SEM. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.