RNAseq analysis of ILC2s purified from uninjured meninges and lung. ILC2s were FACS purified from meninges and lung preparations (DAPI⁻/CD45⁺/Lineage⁻/Thy1.2⁺/ST2⁺ cells), and purified mRNA was sequenced on an Illumina platform. (A) The top 50 genes by mean normalized read counts expressed in meningeal ILC2s (n = 3, each sample five pooled mice). (B) UpSet plot comparing highly expressed genes (expressed >1.5 standard deviations above mean) of meningeal and lung ILC2s. 1,200 highly expressed genes were common to both groups, whereas 219 were specific to lung and 176 specific to meningeal ILC2s. (C) Heat map of significantly altered genes between groups (472 genes; n = 3, each sample five pooled mice; adjusted p-value <0.05). (D and E) Gene sets enriched among differentially expressed genes in lung versus meningeal ILC2s. Histogram (D) and heat maps (E) of select gene sets and their component genes (n = 3, each sample five pooled mice; adjusted p-value <0.05).