Figure 1.
T cell expression of Blimp-1 promotes allergic lung inflammation. Analysis of lung tissue isolated from control (Blimp-1f/f CD4Cre−) or Blimp-1CD4Cre (Blimp-1f/f CD4Cre+) animals i.n. immunized with HDM. (A) PAS staining (scale bar, 100 µm). (B) Percent and absolute number of infiltrating inflammatory cells in the BAL. (C–F) Flow analysis (C and E) and (D and F) percent of Th1 (IFN-γ+ T-bet+), Th2 (GATA3+ IL-13+ or GATA3+ IL-5+) Th17 (Rorγt+ IL-17+) cells and T regulatory cells (FoxP3+) isolated from lungs (gated on Live, CD4+ TCRβ+). (G) Assessment of AHR in unimmunized or HDM-immunized mice. Rn, central airway resistance (Newtonian resistance). Rrs, respiratory system resistance. Mch, methylcholine. Data in A–G are pooled from two or three experiments with 8–13 total mice per group, mean ± SD. Kruskal–Wallis one-way ANOVA (D), Mann–Whitney t test (B and F), or two-way ANOVA (G). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

T cell expression of Blimp-1 promotes allergic lung inflammation . Analysis of lung tissue isolated from control (Blimp-1f/f CD4Cre−) or Blimp-1CD4Cre (Blimp-1f/f CD4Cre+) animals i.n. immunized with HDM. (A) PAS staining (scale bar, 100 µm). (B) Percent and absolute number of infiltrating inflammatory cells in the BAL. (C–F) Flow analysis (C and E) and (D and F) percent of Th1 (IFN-γ+ T-bet+), Th2 (GATA3+ IL-13+ or GATA3+ IL-5+) Th17 (Rorγt+ IL-17+) cells and T regulatory cells (FoxP3+) isolated from lungs (gated on Live, CD4+ TCRβ+). (G) Assessment of AHR in unimmunized or HDM-immunized mice. Rn, central airway resistance (Newtonian resistance). Rrs, respiratory system resistance. Mch, methylcholine. Data in A–G are pooled from two or three experiments with 8–13 total mice per group, mean ± SD. Kruskal–Wallis one-way ANOVA (D), Mann–Whitney t test (B and F), or two-way ANOVA (G). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

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