Functionality of KLRG1⁺ ILCs and NKp46⁺ ILCs in tissues. (A) Representative flow cytometric analysis of intracellular IL-13, IL-17A, IFN-γ, and IL-22 in KLRG1⁺ ILCs, ILC2s, and NKp46⁺ ILCs isolated from NPs, cultured on OP9-DL1 cells in the presence of IL-2 (20 U/ml) and IL-7 (20 ng/ml) with or without IL-1β and IL-23 (20 ng/ml each) for 7 d, and subsequently stimulated by PMA/ionomycin for 3 h. (B) Representative flow cytometric analysis of intracellular IL-13, IL-17A, IFN-γ, and IL-22 in NKp44⁻NKp46⁺ ILC3s and NKp44⁺NKp46⁺ ILC3s isolated from tonsils, cultured and stimulated as in A. (C) Quantification of cytokine production by ELISA in culture supernatants from cells stimulated as in A. The concentration is adjusted to 5,000 cells. Data in A and B are representative of at least three donors from three independent experiments.