Unique epigenetic features of KLRG1⁺ ILCs support their immature state. (A) Venn diagram showing the total number of ATAC-seq peaks (n = 26,192, detected in at least two replicate datasets) and the subsets uniquely or substantially more accessible (>2.0-fold) in either ILC subset. (B and C) PCA (B) or hierarchical clustering (C) on the 747 differentially enriched ATAC-seq peaks defined in A. (D) Pathway enrichment analysis using the genes assigned to both sets of differentially enriched ATAC-seq peaks. (E and F) Representative genome browser shots of ATAC-seq signals across selected loci (E); signals in highlighted regions were quantified across the three biological replicate datasets generated (F). Error bars indicate SEM. PC, principal component; ICOS, inducible T cell costimulator; ICOSLG, inducible T cell costimulator ligand; IFNG, interferon gamma; LCR, locus control region.