Microarray analysis of KLRG1⁺ ILCs, NKp46⁺ ILCs, ILC2s, tonsil NKp44⁻ ILC3s, and NKp44⁺ ILC3s. (A) PCA plot of gene expression as determined by microarray in KLRG1⁺ ILCs, ILC2s, NKp46⁺ ILCs, tonsil isolated NKp44⁻ ILC3s, and NKp44⁺ ILC3s; three donors each). (B) Heatmap of significantly different expressed genes associated with ILC2s and ILC3s. (C) Log₂ expression showing the expression values of selected genes. (D) Heatmap of significantly differently expressed genes between KLRG1⁺ ILCs and ILC2s (left) and log₂ expression of HPGDS and CRLF2 (right). (E) Heatmap of expression of genes related to ILC2 exhaustion. (F) Representative histograms of expression of CD62L and CD45RA on KLRG1⁺ ILCs, NKp46⁺ ILCs, and ILC2s. (G) Venn diagram showing genes that are up-regulated in NKp46⁺ ILCs, tonsil NKp44⁻ ILC3s, and tonsil NKp44⁺ ILC3s. (H) Heatmap of significantly different expressed genes between NKp46⁺ ILCs, tonsil NKp44⁻ ILC3s, and tonsil NKp44⁺ ILC3s. Data in F are representative of at least three donors from at least three independent experiments. PC, principal component; HLA-DRA, human leukocyte antigen DR alpha; HLF, hepatic leukemia factor; HPGD, 15-hydroxyprostaglandin dehydrogenase; HPGDS, hematopoietic prostaglandin D synthase; KIT, proto-oncogene, receptor tyrosine kinase; RORA, RAR-related orphan receptor.