Treatment effects of MAPK and NOTCH repression in colon cancer xenografts. (A) Quantification of necrosis in SW480, SW1222, PDX1, and PDX2 colon cancer xenografts after long-term treatment with AZD and/or DBZ or vehicle (Ctrl) as indicated. Data are mean ± SD *, P < 0.05; **, P < 0.01; ***, P < 0.001 by t test; n.s., not significant. n ≥ 3 independent biological replicates. (B) Representative overview micrographs of H&E-stained sections of xenograft tumors after indicated long term treatments. (C) Representative immunostainings for FRA1 and NICD in SW480 xenografts after long term treatment with vehicle (Ctrl), or AZD and DBZ. Arrowheads indicate remaining FRA1- and NICD-positive tumor cells. Bars: 1 mm (B); 25 µm (C).