Therapeutic targeting of MAPK and NOTCH in colon cancer xenografts. (A) Long term treatment effects of AZD, DBZ, their combination, or vehicle (Ctrl) on SW480-, SW1222-, and patient-derived (PDX1 and PDX2) colon cancer xenografts, shown as growth curves (upper panels) and tumor specific survival in Kaplan-Meier plots (lower panels). Data are mean ± SE in growth curves. P-values are log-rank test results in Kaplan-Meier plots. n ≥ 10 independent biological replicates for each treatment group. (B and C) Quantification of immunostaining for Ki67 proliferation index (B) and cleaved (Cl.) caspase-3 (C) in treated xenografts. Error bars are mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by t test. n ≥ 3 independent biological replicates.