Effects of MAPK and NOTCH repression on EMT in colon cancer xenografts. (A) Relative expression levels of selected EMT-related genes in SW480 xenografts after 5 d of treatment with AZD or DBZ. Data are mean and error bars indicate SD. n = 3 independent biological replicates. (B) Immunoblotting for indicated proteins on tumor lysates of SW480 xenografts after 5 d of vehicle (Ctrl), AZD, or DBZ treatment. n ≥ 3 independent biological replicates, 2 of which are shown. (C) Representative immunostainings (left) and quantification of relative staining intensities (% RSI, right) for E-cadherin and Vimentin in SW480 and/or PDX1 xenografts after 5 d of vehicle (Ctrl), AZD, or DBZ treatment. Areas above dotted lines are tumor necrosis. Bars, 25 µm. Error bars are mean ± SD. **, P < 0.01; ***, P < 0.001 by t test; n.s., not significant. n ≥ 3 independent biological replicates.