Impact of MAPK and NOTCH repression on gene expression in colon cancer in vivo. (A) Venn diagram of genes with significantly (P < 0.05) differential expression and two or more fold change in SW480 xenografts that were treated for five consecutive days with AZD or DBZ compared with vehicle treatment (Ctrl). (B) Heat map and unsupervised hierarchical clustering of genes with significantly (P < 0.05) differential expression and two or more fold change (left). Rows represent genes and columns represent biological replicates. Four main clusters are indicated. Hallmark gene sets most enriched in each cluster as determined by GSEA (right). (C and D) GSEA for indicated gene sets using unfiltered gene expression data of DBZ- (C) and AZD-treated (D) tumors compared with controls. ES, enrichment score; NES, normalized enrichment score. (A–D) n = 3 independent biological replicates.